1. INTEGO: Ubu buryo bugamije gutanga inzira zisanzwe zikoreshwa no kurinda ibyumba bito.
2. Umwanya wa porogaramu: Laboratwari yo kwipiminya ibinyabuzima
3. Umuntu ufite inshingano: Umuyobozi mukuru wa QC
4.Guhuza: Ntayo
5. Inganda z'umutekano
Kora ibikorwa bya andeptic kugirango wirinde kwanduza microbial; Abakora bagomba kuzimya itara rya UV mbere yo kwinjira mucyumba cya sterile.
6.Propit
6.1. Icyumba cya sterile kigomba kuba gifite icyumba cyo gukora sterile hamwe nicyumba cya buffer. Isuku yicyumba cyo gukora sterile igomba kugera ku cyiciro cya 10000. Ubushyuhe bwo mu nzu bigomba kubungabungwa kuri 20-24 ° C kandi ubushuhe bigomba kubungabungwa kuri 45-60%. Isuku yintebe isukuye igomba kugera ku ishuri 100.
6.2. Icyumba cya sterile kigomba kuba gifite isuku, kandi birabujijwe rwose kurundanya imyanda kugirango birinde kwanduza.
6.3. Irinde rwose umwanda wibikoresho byose byo kunyeganyega hamwe nitangazamakuru ryumuco. Abanduye bagomba kureka kubikoresha.
6.4. Icyumba cya sterile kigomba kuba gifite ibihano byangiza ibikorwa byakazi, nkibintu 5% bya Cresol, inzoga 70%, 0.1% chlormetine igisubizo, nibindi
6.5. Icyumba cya sterile kigomba guhonyora buri gihe kandi gisukurwa no kutibuka bikwiye kugirango tumenye ko isuku yicyumba cya sterile yujuje ibisabwa.
6.6. Ibikoresho byose, ibikoresho, amasahani nibindi bintu bigomba kuzanwa mucyumba cya sterile kigomba gupfunyika cyane kandi gigandukira uburyo bukwiye.
6.7. Mbere yo kwinjira mu cyumba cya sterile, abakozi bagomba gukaraba intoki cyangwa kwanduza, hanyuma bagahinduka mucyumba kidasanzwe cyakazi, inkweto, amababi yongeye guhinduka hamwe na 70% Ethanol) mbere yo kwinjira mucyumba cya sterile. Kora ibikorwa mucyumba cya bagiteri.
6.8. Mbere yo gukoresha icyumba cya sterile, itara rya ultraviolet mucyumba cya sterile kigomba gufungurwa no gutombora iminota irenze 30, kandi intebe isukuye igomba guhindurwa ngo ivuruke icyarimwe. Igikorwa kimaze kurangira, icyumba cya sterile kigomba gusukurwa mugihe hanyuma gikonja numucyo wa ultraviolet muminota 20.
6.9. Mbere yo kugenzura, gupakira inyuma yikizamini cyikizamini bigomba kubikwa neza kandi ntibigomba gufungurwa kugirango birinde umwanda. Mbere yo kugenzura, koresha imipira 70% yinzozi zo kwanduza hejuru.
6.10. Muri buri gikorwa, hagomba gukorwa nabi kugirango bigenzure kwizerwa kubikorwa byikirere.
6.11. Mugihe ukurura amazi ya bagiteri, ugomba gukoresha umupira wo guswera kugirango uyakire. Ntukore ku munwa wawe.
6.12. Urushinge rwa InouLlation rugomba gucika intege kubera urumuri mbere na nyuma ya buri gukoresha. Nyuma yo gukonja, umuco urashobora gukubitwa.
6.13. Ibyatsi, ibizamini by'ibizamini, ibizamini bya Petri n'ibindi bikoresho birimo amazi ya bagiteri bigomba kwambara mu indogobe yo guswera zirimo kwanduzwa 5% yo kwanduza, hanyuma ikurwa no kwoza nyuma yamasaha 24.
6.14. Niba hari amazi ya bagiteri yamenetse kumeza cyangwa hasi, ugomba guhita usuka umuti wa karindwi ya 5% cyangwa 3% kuri 3% kumafaranga yanduye byibuze iminota 30 mbere yo kuyifata Iyo imirimo y'akazi n'ingofero yanduye n'amazi ya bagiteri, bagomba guhitanwa ako kanya no gukaraba nyuma yo kumena igitutu kinini.
6.15. Ibintu byose birimo bagiteri nzima bigomba kwanduzwa mbere yo kwoza munsi yigituba. Birabujijwe rwose kwanduza umwanda.
6.16. Umubare wabakoloni mucyumba cya sterile kigomba kugenzurwa buri kwezi. Hamwe nintebe isukuye irakinguye, fata ibiryo bya petrile petrile hamwe na diameter yimbere ya mm 90, kandi biteye ubwoba ml 15 yintungamubiri za ADREETER AGAR Igitekerezo cya AGAR gishonga kandi gikonjesha hafi 45 ° C. Nyuma yo gukomera, ubishyire hejuru kuri 30 kugeza kuri 35 imishito yamasaha 48 muri ℃ incubator. Nyuma yo kwerekana amayeri, fata amasahani 3 kuri 5 hanyuma ubishyire ibumoso, hagati n'uburenganzira bw'akazi. Nyuma yo gufungura igifuniko no kubashyira muminota 30, ubashyire hejuru muri 30 kugeza 35 ° C incubator kumasaha 48 barabakuramo. Suzuma. Impuzandengo ya bagiteri zitandukanye ku isahani mu cyiciro cya 100 gisukuye ntigishobora kurenga 1 Niba imipaka irenze, icyumba cya sterile kigomba kwanduzwa neza kugeza ubugenzuzi bwasubiwemo bwujuje ibisabwa.
7. Reba ku gice (uburyo bwo kugenzura ubugenzuzi) muri "Uburyo bwo kugenzura ibiyobyabwenge" na "Ubushinwa busanzwe bwo gukora bwo kugenzura ibiyobyabwenge".
8. Ishami rikwirakwizwa: Ishami rishinzwe gucunga ubuziranenge
Isuku yubuyobozi bwa tekiniki:
Nyuma yo kubona ibidukikije bito n'ibikoresho biryoshye, tugomba gukomeza leta itoroshye kugirango twige mikorobe idasanzwe cyangwa ikoreshwa mubikorwa byabo. Bitabaye ibyo, mikorobe zitandukanye ziva hanze irashobora kuvanga byoroshye. Ibintu byo kuvanga microrozism idafite ishingiro hanze yitwa kwanduza bagiteri muri mikorobiologiya. Kwirinda kwanduza nubuhanga bukomeye mubikorwa bya microbiologiya. Kugereranya burundu kumuboko umwe no kwirinda kwanduza kurundi ni ibintu bibiri bya tekinike ya asseptic. Byongeye kandi, tugomba gukumira mikorobe ziga, cyane cyane mikorobe ya patogari cyangwa mikorobe y'amafaranga y'ibidukikije bitabaho muri kamere, guhungabanya ibikoresho byacu bigeragejwe mu bidukikije hanze. Kuri izo ntego, muri mikorobiologiya, hari ingamba nyinshi.
Icyumba cya sterile mubisanzwe nicyumba gito cyashyizweho bidasanzwe muri laboratoire ya microbiologiya. Irashobora kubakwa hamwe nimpapuro nikirahure. Agace kagomba kuba nini cyane, nko muri metero kare 4-5, kandi uburebure bugomba kuba nka metero 2.5. Icyumba cya buffer kigomba gushyirwaho hanze yicyumba cya sterile. Umuryango wicyumba cya buffer hamwe numuryango wicyumba cya sterile ntigikwiye guhura nicyerekezo kimwe cyo gukumira umwuka wo kuzana indege zinyuranye. Icyumba cya sterile nicyumba cya buffer kigomba kuba cyiza. Ibikoresho byo mu mazu bigomba kugira ibikoresho byo kurwara. Igorofa n'inkuta z'icyumba cya sterile kigomba kuba cyoroshye, biragoye kubika umwanda kandi byoroshye gusukura. Akazi kagomba kuba urwego. Icyumba cya sterile no mucyumba cya buffer gifite amatara ya ultraviolet. Amatara ya ultraviolet mucyumba cya sterile ni metero 1 kure yakazi. Abakozi binjira mucyumba cya sterile bagomba kwambara imyenda yanduye kandi ingofero.
Kugeza ubu, ibyumba bito ahanini bibaho muri mikorobekiri, mugihe laboratoire rusange Koresha intebe isukuye. Imikorere nyamukuru yintebe isukuye ni ugukoresha ibikoresho byumuriro kugirango ukureho uduce duto duto duhagaze muri mikorobe kumurimo. Igikoresho cyamashanyarazi cyemerera umwuka kunyura hejuru ya Hepa hanyuma hanyuma winjire hejuru yakazi, kugirango akazi gahora kagenzurwa nindege itemba. Byongeye kandi, hariho umwenda wihuta cyane kuruhande hafi yo kwirinda umwuka wo hanze kwinjira.
Ahantu hamwe nibihe bitoroshye, agasanduku ka sterile yimbaho birashobora kandi gukoreshwa aho kuba intebe isukuye. Agasanduku k'ibintu kafite imiterere yoroshye kandi biroroshye kwimuka. Hano hari ibyobo bibiri imbere yisanduku, bikaranzwe no gusunika imiryango ikurura iyo idakora. Urashobora kwagura amaboko mugihe cyo gukora. Igice cyo hejuru cyimbere gifite ibirahuri kugirango byorohereze imikorere yimbere. Hano hari itara rya ultraviolet imbere murigasanduku, kandi ibikoresho na bagiteri birashobora gushyirwa munzu nto kuruhande.
Ubuhanga bwo gukora bungeptike ntabwo bugira uruhare runini mubushakashatsi bwa microbiologiya no gusaba, ariko nabwo ikoreshwa cyane mubi biotechnologies nyinshi. Kurugero, tekinoroji ya Transenic, Tekinoroji ya Monoclonal Antibods, nibindi
Igihe cya nyuma: Werurwe-06-2024