1. Intego: Ubu buryo bugamije gutanga uburyo busanzwe bwo gukora ibikorwa byo kutagira ibisebe no kurinda ibyumba bidafite ibisebe.
2. Aho ikoreshwa: Laboratwari yo gupima ibinyabuzima
3. Umuntu ushinzwe: Umuyobozi w’isuzuma rya QC
4. Ibisobanuro: Nta na kimwe
5. Ingamba zo kwirinda
Kora ibikorwa byo kwirinda kwandura udukoko mu buryo butangiza ibidukikije (aseptic operations) kugira ngo wirinde kwanduza mikorobe; abakora bagomba kuzimya itara rya UV mbere yo kwinjira mu cyumba cy’imyanda.
6. Uburyo bwo gukora
6.1. Icyumba cy’ubutabire kigomba kuba gifite icyumba cyo gukoreramo ubutabire n’icyumba cyo gufunguriramo. Isuku y’icyumba cyo gukoreramo ubutabire igomba kugera ku rwego rwa 10000. Ubushyuhe bwo mu nzu bugomba kuguma kuri 20-24°C n’ubushuhe bugomba kuguma kuri 45-60%. Isuku y’intebe isukuye igomba kugera ku rwego rwa 100.
6.2. Icyumba gisukuye kigomba kubungabungwa isuku, kandi birabujijwe cyane kurunda imyanda kugira ngo hirindwe kwanduzwa.
6.3. Kurinda byimazeyo kwanduza ibikoresho byose byo koza urubyaro n'ibikoresho byo gukwirakwiza ibiribwa. Abanduye bagomba kureka kubikoresha.
6.4. Icyumba gisukuye kigomba kuba gifite imiti yica udukoko ikoreshwa mu kwica udukoko, nka 5% ya cresol, 70% ya alcool, 0.1% ya chlormethionine, nibindi.
6.5. Icyumba cyanduye kigomba gusukurwa buri gihe no gusukurwa hakoreshejwe imiti yica udukoko ikwiye kugira ngo isuku y’icyumba cyanduye yuzuze ibisabwa.
6.6. Ibikoresho byose, ibikoresho, amasahani n'ibindi bikoresho bigomba kujyanwa mu cyumba gisukuye bigomba gupfunyikwa neza no gusukurwa hakoreshejwe uburyo bukwiye.
6.7. Mbere yo kwinjira mu cyumba cy’umuti utetse, abakozi bagomba gukaraba intoki zabo n’isabune cyangwa umuti wica udukoko, hanyuma bakambara imyenda yihariye y’akazi, inkweto, ingofero, udupfukamunwa n’uturindantoki mu cyumba cy’umuti utetse (cyangwa kongera guhanagura intoki zabo na 70% ethanol) mbere yo kwinjira mu cyumba cy’umuti utetse. Bakora ibikorwa byo kubaga mu cyumba cy’umuti utetse.
6.8. Mbere yo gukoresha icyumba cy’ubuziranenge, itara rya ultraviolet riri mu cyumba cy’ubuziranenge rigomba gucanwa kugira ngo rihishwe imirasire no gucibwa burundu mu gihe kirenze iminota 30, kandi intebe isukuye igomba gucanwa kugira ngo umwuka uhuhe icyarimwe. Nyuma y’uko igikorwa kirangiye, icyumba cy’ubuziranenge kigomba gusukurwa ku gihe hanyuma kigashyirwamo ubuziranenge n’urumuri rwa ultraviolet mu gihe cy’iminota 20.
6.9. Mbere yo kugenzura, ipaki yo hanze y'icyitegererezo cy'ikizamini igomba kubikwa neza kandi ntigomba gufungurwa kugira ngo hirindwe kwandura. Mbere yo kugenzura, koresha udupira twa 70% by'ipamba kugira ngo wica udukoko two hanze.
6.10. Muri buri gikorwa, hagomba gukorwa igenzura ridafite ingaruka mbi kugira ngo harebwe niba ibikorwa byo kutagira aside bihamye ari ingirakamaro.
6.11. Mu gihe urimo gukurura amazi ya bagiteri, ugomba gukoresha agapira ko kuyakurura kugira ngo uyakurure. Ntugakore ku gahu k'umunwa wawe.
6.12. Urushinge rwo gutera umuti rugomba gusukurwa n'umuriro mbere na nyuma ya buri ikoreshwa. Nyuma yo gukonjesha, umuti ushobora guterwa umuti.
6.13. Uduti, imiyoboro yo gupima, amasahani ya petri n'ibindi bikoresho birimo amazi ya bagiteri bigomba kwinjizwa mu ndobo yo gusukura irimo umuti wa Lysol wa 5% kugira ngo bisukurwe, hanyuma bikurwemo bikozwe nyuma y'amasaha 24.
6.14. Niba hari amazi ya bagiteri yamenetse ku meza cyangwa hasi, ugomba guhita usuka umuti wa aside karuboliki ya 5% cyangwa Lysol ya 3% ahantu handuye byibuze iminota 30 mbere yo kuhavura. Iyo imyenda n'ingofero byandujwe n'amazi ya bagiteri, bigomba guhita bikurwaho hanyuma bikaraba nyuma yo gusukurwa hakoreshejwe umwuka mwinshi.
6.15. Ibintu byose birimo bagiteri nzima bigomba gusukurwa mbere yo kozwa munsi y'amazi. Birabujijwe cyane kwanduza imyanda.
6.16. Umubare w'amatsinda mu cyumba cy'imyanda ugomba kugenzurwa buri kwezi. Iyo intebe isukuye ifunguye, fata amasahani menshi ya petri yanduye afite umurambararo w'imbere wa mm 90, hanyuma ushyiremo mililitiro 15 z'intungamubiri za agar zashongeshejwe kandi zikonjeshwa kugeza kuri dogere selisiyusi 45. Nyuma yo gukomera, shyira hejuru ku gipimo cya dogere selisiyusi 30 kugeza kuri 35. Umaze kwerekana ko nta ndwara, fata amasahani 3 kugeza kuri 5 uyashyire ibumoso, hagati n'iburyo bw'aho ukorera. Nyuma yo gufungura igipfundikizo no kuyashyira ahagaragara mu gihe cy'iminota 30, uyashyire hejuru mu gipimo cya dogere selisiyusi 30 kugeza kuri 35 mu gihe cy'amasaha 48 hanyuma uyakuremo. Gerageza gusuzuma. Umubare mpuzandengo wa bagiteri zitandukanye ku isahani mu gace gasukuye ka 100 ntugomba kurenza ikoloni 1, kandi umubare mpuzandengo mu cyumba gisukuye ka 10000 ntugomba kurenza amatsinda 3. Iyo igihe ntarengwa kirenze, icyumba cyanduye kigomba gusukurwa neza kugeza igihe isuzuma risubiwemo ryujuje ibisabwa.
7. Reba igice (Uburyo bwo Gusuzuma Isuku y'Imiti) muri "Uburyo bwo Gusuzuma Isuku y'Imiti" na "Uburyo Busanzwe bwo Gusuzuma Imiti mu Bushinwa".
8. Ishami rishinzwe gukwirakwiza: Ishami rishinzwe gucunga ubuziranenge
Ubuyobozi bwa tekiniki bwo gusukura icyumba:
Nyuma yo kubona ibidukikije bidafite umwanda n'ibikoresho bidafite umwanda, tugomba kugumana imiterere yabyo kugira ngo twige mikorobe runaka izwi cyangwa dukoreshe imikorere yazo. Bitabaye ibyo, mikorobe zitandukanye zo hanze zishobora kwivanga byoroshye. Uburyo bwo kuvanga mikorobe zidafite akamaro ziturutse hanze byitwa kwanduza bagiteri muri mikorobe. Kurinda kwandura ni uburyo bw'ingenzi mu kazi ka mikorobe. Guhagarika burundu ku ruhande rumwe no gukumira kwandura ku rundi ruhande ni ibintu bibiri bigize uburyo bwo gusesereza. Byongeye kandi, tugomba gukumira mikorobe ziri kwigwa, cyane cyane mikorobe zitera indwara cyangwa mikorobe zahinduwe mu buryo bwa genetiki zitabaho mu bidukikije, kuva mu bikoresho byacu by'igerageza tukajya mu bidukikije byo hanze. Kuri izi ntego, muri mikorobe, hari ingamba nyinshi.
Icyumba cy’imyanda gikunze kuba icyumba gito gikorerwa muri laboratwari ya mikorobiyoloji. Gishobora kubakwa hakoreshejwe impapuro n'ibirahure. Ahantu hatagomba kuba hanini cyane, hafite metero kare 4-5, kandi uburebure bugomba kuba metero 2.5. Icyumba cy’imyanda kigomba gushyirwa hanze y’icyumba cy’imyanda. Urugi rw’icyumba cy’imyanda n’umuryango w’icyumba cy’imyanda ntibigomba kwerekeza mu cyerekezo kimwe kugira ngo hirindwe ko umwuka winjira mu kirere utazana bagiteri zitandukanye. Icyumba cy’imyanda n’icyumba cy’imyanda byombi bigomba kuba bidafite umwuka. Ibikoresho byo guhumeka mu nzu bigomba kuba bifite ibikoresho bisukura umwuka. Hasi n’inkuta by’icyumba cy’imyanda bigomba kuba byoroshye, bigoye kubikamo umwanda kandi byoroshye gusukura. Ahantu ho gukorera hagomba kuba haringaniye. Icyumba cy’imyanda n’icyumba cy’imyanda byombi bifite amatara ya ultraviolet. Amatara ya ultraviolet mu cyumba cy’imyanda ari kure ya metero 1 uvuye aho bakorera. Abakozi binjira mu cyumba cy’imyanda bagomba kwambara imyenda n’ingofero byasizwe imiti.
Muri iki gihe, ibyumba bisukuye bikunze kuba mu nganda zikora mikorobe, mu gihe laboratwari rusange zikoresha intebe isukuye. Inshingano nyamukuru y'intebe isukuye ni ugukoresha igikoresho gisukuye gitemba umwuka kugira ngo gikureho ivumbi rito ritandukanye harimo na mikorobe ku buso bw'akazi. Igikoresho gikoresha amashanyarazi cyemerera umwuka kunyura muri hepa filter hanyuma ukinjira ku buso bw'akazi, ku buryo buso bw'akazi buhora bugenzurwa n'umwuka usukuye utemba. Byongeye kandi, hari agatambaro k'umwuka wihuta cyane ku ruhande rwegereye inyuma kugira ngo wirinde ko umwuka wo hanze wa bagiteri winjira.
Mu bice bigoye, udusanduku tw’ibiti twangiritse dushobora gukoreshwa aho gukoresha intebe isukuye. Agasanduku katarinzwe gafite imiterere yoroshye kandi biroroshye kwimuka. Hari imyobo ibiri imbere y’agasanduku, ifunze n’inzugi zisunikwa iyo zidakora. Ushobora kurambura amaboko yawe mu gihe cyo gukora. Igice cyo hejuru cy’imbere gifite ikirahuri kugira ngo cyoroshye gukora imbere. Hari itara rya ultraviolet imbere mu gasanduku, kandi ibikoresho na bagiteri bishobora gushyirwa mu muryango muto ku ruhande.
Ubuhanga bwo gukoresha aseptic ntabwo bugira uruhare runini mu bushakashatsi no mu mikorere ya mikorobe gusa, ahubwo bunakoreshwa cyane muri biotechnologies nyinshi. Urugero, ikoranabuhanga rya transgenic, ikoranabuhanga rya monoclonal antibody, nibindi.
Igihe cyo kohereza: Werurwe-06-2024