1. Intego: Ubu buryo bugamije gutanga uburyo busanzwe bwo gukora aseptic no kurinda ibyumba bitagira ingano.
2. Igipimo cyo gusaba: laboratoire yo gupima ibinyabuzima
3. Umuntu Ushinzwe: Ikizamini cya QC Umugenzuzi
4.Ibisobanuro: Ntayo
5. Kwirinda umutekano
Kora cyane ibikorwa bya aseptic kugirango wirinde kwanduza mikorobe;abakoresha bagomba kuzimya itara rya UV mbere yo kwinjira mucyumba cya sterile.
6.Ibikorwa
6.1.Icyumba cya sterile kigomba kuba gifite icyumba cyo gukoreramo cya sterile hamwe nicyumba cya buffer.Isuku yicyumba cyo gukoreramo sterile igomba kugera mu cyiciro 10000. Ubushyuhe bwo mu nzu bugomba kuguma kuri 20-24 ° C naho ubuhehere bugakomeza kubikwa kuri 45-60%.Isuku yintebe isukuye igomba kugera mu cyiciro cya 100.
6.2.Icyumba cya sterile kigomba guhorana isuku, kandi birabujijwe rwose kurunda imyanda kugirango wirinde kwanduza.
6.3.Irinde rwose kwanduza ibikoresho byose byo kuboneza urubyaro nibitangazamakuru byumuco.Abanduye bagomba guhagarika kubikoresha.
6.4.Icyumba cya Sterile kigomba kuba gifite ibikoresho byangiza imiti, nka 5% yumuti wa cresol, 70% alcool, 0.1% chlormethionine, nibindi.
6.5.Icyumba cya sterile kigomba guhora gisukurwa kandi kigasukurwa hifashishijwe imiti yica udukoko kugira ngo isuku yicyumba cya sterile yujuje ibisabwa.
6.6.Ibikoresho byose, ibikoresho, amasahani nibindi bintu bigomba kwinjizwa mucyumba cya sterile bigomba kuzingirwa cyane kandi bigahinduka hakoreshejwe uburyo bukwiye.
6.7.Mbere yo kwinjira mucyumba cya sterile, abakozi bagomba gukaraba intoki bakoresheje isabune cyangwa yangiza, hanyuma bagahindura imyenda idasanzwe yakazi, inkweto, ingofero, masike na gants mu cyumba cya buffer (cyangwa bongera guhanagura intoki hamwe na 70% Ethanol) mbere yo kwinjira mucyumba cya sterile.Kora ibikorwa mubyumba bya bagiteri.
6.8.Mbere yo gukoresha icyumba cya sterile, itara rya ultraviolet mucyumba cya sterile rigomba gukingurwa kugirango rirasiyo na sterisile mu gihe kirenze iminota 30, kandi intebe isukuye igomba gukingurwa kugirango umwuka uhuhira icyarimwe.Igikorwa kimaze kurangira, icyumba cya sterile kigomba gusukurwa mugihe gikwiye hanyuma kigahagarikwa numucyo ultraviolet muminota 20.
6.9.Mbere yo kugenzura, ibipapuro byo hanze byikitegererezo bigomba guhorana neza kandi ntibigomba gukingurwa kugirango birinde umwanda.Mbere yo kugenzura, koresha 70% imipira yinzoga kugirango wanduze hejuru.
6.10.Muri buri gikorwa, hagomba gukorwa igenzura ribi kugirango harebwe niba ibikorwa bya aseptic byizewe.
6.11.Mugihe winjiza amazi ya bagiteri, ugomba gukoresha umupira wokunywa.Ntukore ku byatsi ukoresheje umunwa wawe.
6.12.Urushinge rwo gukingira rugomba guhagarikwa n'umuriro mbere na nyuma yo gukoreshwa.Nyuma yo gukonja, umuco urashobora guterwa.
6.13.Ibyatsi, imiyoboro yipimisha, ibyokurya bya petri nibindi bikoresho birimo amazi ya bagiteri bigomba gushirwa mu ndobo ya sterilisation irimo 5% ya Lysol yumuti wo kwanduza, hanyuma bikavamo no kozwa nyuma yamasaha 24.
6.14.Niba hari amazi ya bagiteri yamenetse kumeza cyangwa hasi, ugomba guhita usuka 5% ya acide karbolic cyangwa 3% Lysol kumwanya wanduye byibuze muminota 30 mbere yo kuyivura.Iyo imyenda y'akazi n'ingofero byandujwe n'amazi ya bagiteri, bigomba guhita bikurwaho hanyuma bigakaraba nyuma yo guhagarika umwuka mwinshi.
6.15.Ibintu byose birimo bagiteri nzima bigomba kwanduzwa mbere yo kozwa munsi ya kanda.Birabujijwe rwose kwanduza umwanda.
6.16.Umubare wabakoloni mucyumba cya sterile ugomba kugenzurwa buri kwezi.Hamwe n'intebe isukuye ifunguye, fata ibyokurya byinshi bya petri sterile bifite diametre y'imbere ya mm 90, hanyuma ushiremo inshinge zigera kuri ml 15 z'intungamubiri z'intungamubiri za agar zashongeshejwe hanyuma zikonja kugeza kuri 45 ° C.Nyuma yo gukomera, shyira hejuru ya 30 kugeza 35 Incubate kumasaha 48 muri ℃ incubator.Nyuma yo kwerekana ubugumba, fata amasahani 3 kugeza kuri 5 hanyuma uyashyire ibumoso, hagati na iburyo bwumwanya wakazi.Nyuma yo gufungura igifuniko no kubishyira ahagaragara muminota 30, ubishyire hejuru muri incubator ya 30 kugeza 35 ° C mumasaha 48 hanyuma ubisohokane.gusuzuma.Impuzandengo ya bagiteri zitandukanye ziri ku isahani mu cyiciro cy’isuku 100 ntishobora kurenza koloni 1, kandi impuzandengo yo mu cyumba cy’isuku 10000 ntishobora kurenga koloni 3.Niba imipaka irenze, icyumba cya sterile kigomba kwanduzwa neza kugeza ubugenzuzi bwujuje ibisabwa.
7. Reba ku gice (Uburyo bwa Sterility Inspection Method) muri "Uburyo bwo Kugenzura Ibiyobyabwenge" na "Ubushinwa bukoresha uburyo bwo kugenzura ibiyobyabwenge".
8. Ishami rishinzwe gukwirakwiza: Ishami rishinzwe gucunga ubuziranenge
Isuku yubuyobozi bwa tekinike:
Nyuma yo kubona ibidukikije bidafite ibikoresho, tugomba gukomeza kuba sterile kugirango twige mikorobe runaka izwi cyangwa dukoreshe imirimo yabo.Bitabaye ibyo, mikorobe zitandukanye ziva hanze zirashobora kuvanga byoroshye. Ikintu cyo kuvanga mikorobe idafite akamaro kiva hanze cyitwa kwanduza bagiteri muri mikorobi.Kwirinda kwanduza ni tekinike ikomeye mubikorwa bya mikorobi.Kurandura burundu kuruhande rumwe no kwirinda kwanduza kurundi ruhande ni ibintu bibiri bya tekinike ya aseptic.Byongeye kandi, tugomba gukumira mikorobe iri kwigwa, cyane cyane mikorobe itera indwara cyangwa mikorobe ikozwe na genetique itabaho muri kamere, guhunga ibintu byabigenewe byinjira mubidukikije.Kubwizo ntego, muri microbiologiya, hariho ingamba nyinshi.
Icyumba cya Sterile mubusanzwe ni icyumba gito cyashyizweho muri laboratoire ya microbiology.Irashobora kubakwa kumpapuro nikirahure.Ubuso ntibukwiye kuba bunini cyane, metero kare 4-5, n'uburebure bugomba kuba nka metero 2,5.Icyumba cya buffer kigomba gushyirwaho hanze yicyumba cya sterile.Urugi rwicyumba cya buffer n umuryango wicyumba cya sterile ntigomba guhura nicyerekezo kimwe kugirango wirinde ko umwuka uzana bagiteri zitandukanye.Icyumba cya sterile hamwe nicyumba cya buffer bigomba kuba byoroshye.Ibikoresho byo guhumeka mu nzu bigomba kugira ibikoresho byo kuyungurura umwuka.Igorofa n'inkuta z'icyumba cya sterile bigomba kuba byoroshye, bigoye kubika umwanda kandi byoroshye kubisukura.Ubuso bwakazi bugomba kuba buringaniye.Icyumba cya sterile hamwe nicyumba cya buffer gifite amatara ya ultraviolet.Amatara ya ultraviolet mucyumba cya sterile ni metero 1 uvuye kumurimo.Abakozi binjira mucyumba cya sterile bagomba kwambara imyenda n'ingofero.
Kugeza ubu, ibyumba bya sterile bibaho cyane mu nganda za mikorobe, naho laboratoire rusange ikoresha intebe isukuye.Igikorwa nyamukuru cyintebe isukuye nugukoresha ibikoresho byoguhumeka ikirere cya laminar kugirango ukureho umukungugu muto utandukanye harimo na mikorobe ku kazi.Igikoresho cyamashanyarazi cyemerera umwuka kunyura muri hepa muyungurura hanyuma ukinjira hejuru yakazi, kugirango ubuso bwakazi burigihe bugumane kugenzurwa numwuka utemba.Byongeye kandi, hari umwenda wihuta wumuyaga kuruhande rwegereye hanze kugirango wirinde umwuka wa bagiteri utinjira.
Ahantu hafite ibihe bigoye, agasanduku ka sterile yimbaho karashobora kandi gukoreshwa aho kuba intebe isukuye.Agasanduku ka sterile gafite imiterere yoroshye kandi byoroshye kwimuka.Hano hari imyobo ibiri imbere yagasanduku, ifunzwe nugusunika-gukurura inzugi iyo idakora.Urashobora kurambura amaboko mugihe cyo gukora.Igice cyo hejuru cyimbere gifite ibirahuri kugirango byorohereze imikorere yimbere.Hano hari itara rya ultraviolet imbere mu gasanduku, kandi ibikoresho na bagiteri birashobora gushirwa mu muryango muto ku ruhande.
Ubuhanga bwo gukora bwa Aseptic ntabwo bugira uruhare runini mubushakashatsi bwa mikorobe no kubukoresha, ahubwo bukoreshwa cyane mubinyabuzima byinshi.Kurugero, tekinoroji ya transgenji, tekinoroji ya antibody ya monoclonal, nibindi.
Igihe cyo kohereza: Werurwe-06-2024